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Procedure for calibration of HPLC (WATERS ALLIANCES)

  • OBJECTIVE
  • To lay down the procedure for calibration of the HPLC (Waters Alliances 2695, Separation Modules).
  • SCOPE
  • To ensure that the calibration procedure of HPLC meets the acceptances criteria. This SOP shall be applicable for the HPLC system. (WATERS Alliances 2695 Separation Modules).
  • RESPONSIBILITY
  • Quality Control Executive/Officer.
  • ACCOUNTABILITY
  • Quality Control Manager.
  • PROCEDURE FOR CLEANING
  • Check that the power supply to the instrument is switched ‘ OFF ‘ before cleaning. Clean the instrument with a clean dry cloth every day. Occasionally wet cloth dipped in dilute soap solution may be used. Precaution has to be taken to clean the instrument immediately with dry cloth to remove the moisture.
  • PROCEDURE FOR CALIBRATION:
  • To perform the calibration of instrument as per procedure given below, follow the operating instruction given in the SOP of the instrument.
  • Following parameters shall be calibrated:
  • For PUMP:
  1. Flow rate Accuracy
  2. For Gradient Performance verification:
  • For Detector:
  1. Wave length accuracy
  2. Linearity Response of Detector.
  • For Auto Sampler:
  1. Injection Repeatability & Percentage carryover.
  2. Linearity of injection volume.
  • For Column Oven:
  1. Oven Temperature.
  • For Pump calibration:
  • 1. Flow rate Accuracy:
  • Select the water as mobile phase and temperature at 25°C.
  • Put the suction filter in to the reservoir containing the water as mobile phase.
  • Set the flow rate at 2.0 ml per minute and open the By-pass system and start purging to remove air bubble from the tubing’s. Decrease the flow rate slowly and stop the pump.
  • Connect the union between pump outlet and detector inlet.
  • Collect the mobile phase at the detector outlet into a cleaned dried 25ml measuring cylinder (previously weight) for known time i.e. at 0.5ml/min for 10 min and calculate the flow rate /min by given formula.
  • Vol. Dispense in the selected time =
  • (Wt of filled Measuring Cylinder(W2) – Wt of empty Measuring Cylinder (W1) ) / (Wt /ml of water at 25°C  )
  •   Flow rate per min = Volume dispense in selected time / Time.
  • Repeat the above procedure for Flow rate of 1.0ml, 1.5ml and 2.0ml for 5 minutes.
  • Record the observation as per given in Annexure 1.
  • 2.  For Gradient Performance verification:
  • Connect the union between pump outlet and detector inlet.
  • Place the suction filter assembly of gradient line A & B in to the solvent reservoir containing methanol (100%).
  • Place the suction filter assembly of gradient line C & D in to the solvent reservoir containing 5.6mg/Lit. solution of Propyl paraben in methanol.
  • Prime the pump.
  • Fill the gradient table parameter as mention below:
Time Flow %A %B %C %D Curve
Initial 2.0 50 50 0 0
2.0 2.0 0 0 50 50 11
6.0 2.0 50 50 0 0 11
10.0 2.0 45 45 10 0 11
12.0 2.0 50 50 0 0 11
14.0 2.0 45 45 0 10 11
16.0 2.0 50 50 0 0 11
18.0 0.0 50 50 0 0 11
  • Set the detector wavelength to 254 nm.
  • Save the method set as GPV_ MS.
  • Set run time for 18 minutes and Inject 20ml methanol as a sample.
  • Check the obtained chromatogram for gradient steps.
  • Process the GPV run with a processing method, to obtain the % of the full scale Peak.
  • Save the result and generate a repot method that gives the GPV %.
  • %GPV should be 10% ±0.5.
  • FOR AUTO SAMPLER:
  • 1. Injection Repeatability & % Carryover:
  • Chromatographic condition:
  1. Wave length: 254 nm
  2. Mobile phase: Methanol: water (85:15) (isocratic)
  3. Flow rate: 1ml /min.
  4. Test Sample: Naphthalene (26mg/100ml in methanol)
  5. Injection Volume: 10µl.
  6. Column: symmetry C18 5µ: 150 X 4.6 mm
  • Run the system as per the above chromatographic Condition. Wait until the base line stabilizes. Prepare a sample set method for the following injections
  1. 10µl methanol as a blank.
  2. 10µl test in 6 replicates
  3. 10µl methanol as a blank for carryover
  • Process the acquired data and calculate the % RSD for Retention time & Peak area.
  • Calculate the % carry over (area of sample in blank for carry over/ average area of sample in test replicates x100).
  • Record the observation as per given in Annexure I.
  • 2. Linearity of Injection Volume:
  • Run the system as per the above chromatographic Condition given in step 8.1.1. Wait until the base line stabilizes, than inject 10µl methanol as a blank.
  • Inject 10µl, 20µl, 30µl 40µl and 50µl. of test sample in duplicate.
  • Record the observation as per given in Annexure I. Plot the graph of Injection volume vs Response (Peak area).
  • For Detector:
  • 1. Wave length Accuracy:
  • Chromatographic condition:
  1. Wave length: 250nm to 266nm at the interval of 2nm.
  2. Mobile phase: Methanol
  3. Flow rate: 1ml /min.
  4. Test Sample: Uracil (20 ppm in methanol).
  5. Injection Volume: 20µl.
  6. Column: symmetry C18 5µ: 150 X 4.6 mm.
  • Run the system as per the above chromatographic Condition, Wait until the base line stabilizes, then inject 20µl methanol as a blank.
  • Inject 20µl of test sample in duplicate at the wavelength of 250 nm.
  • Repeat the process at the wavelength of 252 nm to 266 nm at the interval of 2 nm.
  • Process and save the acquired data and record the response at each wavelength in calibration table of Annexure I.
  • Compare the results and measure the wavelength maximum in terms of maximum response of area.
  • 2.Linearity of Detector Response.
  • Prepare the test sample of Uracil 2 ppm to 10 ppm at the interval of 2 ppm in methanol.
  • Run the system as per the chromatographic condition given in step 9.1.1, Wait until the base line stabilizes, then inject 20µl methanol as a blank.
  • Separately inject each 2 ppm to 10 ppm solution induplicate and take the mean of the solution for linearity study.
  • Plot the graph for Concentration VS Response (Peak area) and calculate the Coefficient of regression.
  • Record the observation as per given in Annexure I.
  • For Column Oven: 
  1. Oven Temperature:
  • Set the column oven to 40°C.
  • Place the probe of thermometer inside the column oven.
  • Note down the initial temperature displayed on the instrument.
  • Note down the temperature displayed by the probe.
  • Allow the column oven to stand for 30min. and note down the temp.
  • Repeat the above procedure similarly for Temperature 50°C and 60°C.
  • Record the instrument displayed temperature and thermometer reading in annexure –I.
  • Enter the details in respective instrument log book.
  • Acceptances criteria:
MODULE PARAMETER STANDARD TOLERANCES FREQUENCY
PUMP I) Flow rate Accuracy By measurement of dispensed volume in specified time. ±2% of Set flow. QUATERLY
II) For Gradient Performances Verification: % Height at each step. 10% ±0.5% QUATERLY
Detector I) Wave length accuracy Maximum response of Uracil at different wavelength. 258nm ±2nm QUATERLY
II) Linearity Response of Detector. Response of Uracil at different concentration 2-ppm to 10 ppm. Regression Coefficient NLT 0.99 QUATERLY
Auto Sampler I) Injection Repeatability & Percentage carryover. By 26 mg / 100 ml Naphthalene in methanol. RSD NMT 0.5% & % Carryover NMT 0.01% QUATERLY
II) Linearity of injection volume. By different injection volume of same concentration. Regression Coefficient NLT 0.99 QUATERLY
Column Oven

 

Oven Temperature. By calibrated thermometer at different temperature i.e. 40°C, 50°C, 60°C. ± 2°C at Set temperature. QUATERLY
± 0.5°C between thermometer reading and instrument displayed value.
  • Forms and Records (Annexures)
  • CALIBRATION OF HPLC – WATERS [ALLIANCE] – Annexure-1
  • Distribution
  • Master copy –  Quality Assurance
  • Controlled copies- Quality Assurance, Production, Quality Control.engineering
  • History
    Date Revision Number Reason for Revision
    00 New SOP

 

Annexure-1

CALIBRATION OF HPLC – WATERS [ALLIANCE]

QUALITY ASSURANCE DEPARTMENT

INSTRUMENT CALIBRATION FORMAT – HIGH PERFORMANCE LIQUID CHROMATOGRAPH

LOCATION   CALIBRATION PROCEDURE NO.
MANUFACTURED BY   INSTRUMENT ID. NO.
MODEL NO.   EFFECTIVE DATE

CLEANLINESS OF EQUIPMENT / INSTRUMENT BEFORE CALIBRATION:  SATISFACTORY/ NOT SATISFACTORY

MODULE: PUMP

  1. FLOW RATE ACCURACY:

STOP WATCH USED:

Flow rate set ml/min (F) Time set

Min (T)

Wt of empty Measuring cylinder

(W1) g

Wt of filled Measuring cylinder

(W2) g

Vol. dispense (V)

V = (W2-W1) / Wt per ml of water At 25°C

Flow per min

=  V / T

Tolerance

±0.2 %

0.5 10 0.49 – 0.51
1.0 5 0.98 – 1.02
1.5 5 1.47 – 1.53
2.0 5 1.96 – 2.04

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

2. Gradient Performances Verification:

S.NO. Peak name Height Acceptance Criteria
1. Full scale 10 %± 0.5
2. A / B / C
3. A / B / D

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

 

MODULE: DETECTOR

1.Wave Length Accuracy:

Standard Uracil used (B. No. / Lot No): _______________________

Standard dilution:

Wave length

(nm)

Replicate Injection RT of Uracil Observation Wavelength maximum observed (nm) Acceptances Criteria
Peak Area Mean
250 1 258 ± 2 nm
2
252 1
2
254 1
2
256 1
2
258 1
2
260 1
2
262 1
2
264 1
2
266 1
2

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

  1. Linearity Responses of Detector:

Standard Uracil used (B. No. / Lot No):_______________________

Standard dilution:

Conc. of Uracil

(ppm)

Replicate Injection RT of Uracil Observation Correlation coefficient Acceptances Criteria
Peak Area Mean
2 1 r ≥0.99
2
4 1
2
6 1
2
8 1
2
10 1
2

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

MODULE: AUTO SAMPLER

  1. Injection Repeatability and % Carry Over:

Standard Naphthalene used (B. No. / Lot No):_______________________

Standard dilution:

% Carry over = Area of sample in blank/ Mean Area of sample in tests X 100

Replicate injection RT of Naphthalene Peak Area Acceptances Criteria  %  Carry Over Acceptances Criteria
1 %RSD of RT

is NMT 0.5%

&

% RSD of Area

is NMT 1.0%

 

NMT 0.01%
2
3
4
5
6
Mean
% RSD
%  Carry over blank

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

  1. Linearity of Injection Volume:
Injection Volume 10.0 µl 20.0 µl 30.0 µl 40.0 µl 50.0 µl
Area of injection 1
Area of injection 2
Mean area
Correlation coefficient Acceptance Criteria r ≥0.99

STATUS: ­­­­­­­­­­­­­­­­ PASS/ FAIL

MODULE: COLUMN OVEN

  1. Oven Temperature:

Thermometer Id.No. _______________________

 

S No. Set Temperature

(°C)

Observed Temperature (°C) Difference Acceptances Criteria
Instrument Thermometer
1 40 ± 2°C at each set temperature

&

± 0.5° C between instrument and thermometer readings

2 50
3 60

STATUS:­­­­­­­­­­­­­­­­ PASS/ FAIL                                                      

OPINION:INSTRUMENT IS SUITABLE/ NOT SUITABLE FOR USE.

NEXT CALIBRATION DUE ON: ­­­————————————————————–

Calibrated by:                                                                  Checked by:                                           Approved by:

Date :                                                                               Date :                                                      Date   :

 

 

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