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To lay down the procedure for Motility test by Hanging drop Method.

Motility test by Hanging drop Method

  • vMotility test by Hanging drop Method
  • Objective

To lay down the procedure for Motility test by Hanging drop Method.

  • Scope

This SOP is applicable for Motility test by Hanging drop Method in Microbiology Laboratory of (Pharmaceutical Company Name).

  • Responsibility
    • Microbiologist or above of Microbiology Laboratory: Preparation of SOP.
    • Head – Microbiology section / Nominee: Checking of the SOP.
  • Accountability
    • Head – Quality Control / Nominee: Compliance of SOP.
  • Abbreviations and Definitions

SOP                             : Standard operating procedure

No.                              : Number

QC                              : Quality Control

QA                              : Quality Assurance

SP                                : Specific

  • Procedure
    • Make suspension of the microbial colony.
    • Apply grease or Vaseline around the depression of the hanging-drop slide.
    • Using the inoculating loop, aseptically transfer one drop of the culture to the center of a clean cover slip.
    • Invert the hanging-drop slide and center its well over the drop of culture. Press down on the edge of the cover slip so that grease makes a firm seal.
    • Quickly and carefully turn the slide right up so the hanging drop is suspended in the depression.
    • Do not let the drop fall or touch the bottom of the depression.
    • To examine the hanging drop, first locate its edge in the center of the microscopic field, with the low power objective (10 x) and markedly reduce the light.
    • You will see the edge as a bright wavy line against a gray background.
    • Switch to the High – dry objective (45 x) and refocus on the edge of the drop.
    • The bacteria within the drop will then be in approximate focus and detect them by their movement and by the contrast between them and the suspending media.
    • Differentiate true motility from Brownian movement carefully.
  • Forms and Records (Annexures)
    • Not applicable
  • Distribution
    • Master copy    –       Quality Assurance
    • Controlled copies     – Quality Assurance, Production, Quality Control, Stores, Engineering and Human Resource
  • History:
Date Revision Number
Reason for Revision
00   NEW SOP

 

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